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Plasmid
pET15b 6His 3C SARS-CoV2 Nsp4
Parent Plasmid
pET15b 6His 3C
Expressed
6His 3C SARS-CoV2 Nsp4
DU Number
DU67814
Genbank
MN908947.3
Tag
6His
Species
SARS-CoV-2
Sequence of Insert
View Sequence
GGATCCAAAATTGTGAACAACTGGCTGAAACAGCTGATTAAAGTGACCCTGGTGTTTCTGTTTGTGGCGGCGATTTTTTATCTGATTACCCCGGTGCATGTGATGAGCAAACATACCGATTTTAGCAGCGAAATTATTGGCTATAAAGCGATTGATGGCGGCGTGACCCGCGATATTGCGAGCACCGATACCTGCTTTGCGAACAAACATGCGGATTTTGATACCTGGTTTAGCCAGCGCGGCGGCAGCTATACCAACGATAAAGCGTGCCCGCTGATTGCGGCGGTGATTACCCGCGAAGTGGGCTTTGTGGTGCCGGGCCTGCCGGGCACCATTCTGCGCACCACCAACGGCGATTTTCTGCATTTTCTGCCGCGCGTGTTTAGCGCGGTGGGCAACATTTGCTATACCCCGAGCAAACTGATTGAATATACCGATTTTGCGACCAGCGCGTGCGTGCTGGCGGCGGAATGCACCATTTTTAAAGATGCGAGCGGCAAACCGGTGCCGTATTGCTATGATACCAACGTGCTGGAAGGCAGCGTGGCGTATGAAAGCCTGCGCCCGGATACCCGCTATGTGCTGATGGATGGCAGCATTATTCAGTTTCCGAACACCTATCTGGAAGGCAGCGTGCGCGTGGTGACCACCTTTGATAGCGAATATTGCCGCCATGGCACCTGCGAACGCAGCGAAGCGGGCGTGTGCGTGAGCACCAGCGGtCGCTGGGTGCTGAACAACGATTATTATCGCAGCCTGCCGGGCGTGTTTTGCGGCGTGGATGCGGTGAACCTGCTGACCAACATGTTTACCCCGCTGATTCAGCCGATTGGCGCGCTGGATATTAGCGCGAGCATTGTGGCGGGCGGCATTGTGGCGATTGTGGTGACCTGCCTGGCGTATTATTTTATGCGCTTTCGCCGCGCGTTTGGCGAATATAGCCATGTGGTGGCGTTTAACACCCTGCTGTTTCTGATGAGCTTTACCGTGCTGTGCCTGACCCCGGTGTATAGCTTTCTGCCGGGCGTGTATAGCGTGATTTATCTGTATCTGACCTTTTATCTGACCAACGATGTGAGCTTTCTGGCGCATATTCAGTGGATGGTGATGTTTACCCCGCTGGTGCCGTTTTGGATTACCATTGCGTATATTATTTGCATTAGCACCAAACATTTTTATTGGTTTTTTAGCAACTATCTGAAACGCCGCGTGGTGTTTAACGGCGTGAGCTTTAGCACCTTTGAAGAAGCGGCGCTGTGCACCTTTCTGCTGAACAAAGAAATGTATCTGAAACTGCGCAGCGATGTGCTGCTGCCGCTGACCCAGTATAACCGCTATCTGGCGCTGTATAACAAATATAAATATTTTAGCGGCGCGATGGATACCACCAGCTATCGCGAAGCGGCGTGCTGCCATCTGGCGAAAGCGCTGAACGATTTTAGCAACAGCGGCAGCGATGTGCTGTATCAGCCGCCGCAGACCAGCATTACCAGCGCGGTGCTGCAGtgaGCGGCCGC
Amino Acid Sequence
View Sequence
MGSSHHHHHHSSGLEVLFQGPGSKIVNNWLKQLIKVTLVFLFVAAIFYLITPVHVMSKHTDFSSEIIGYKAIDGGVTRDIASTDTCFANKHADFDTWFSQ
RGGSYTNDKACPLIAAVITREVGFVVPGLPGTILRTTNGDFLHFLPRVFSAVGNICYTPSKLIEYTDFATSACVLAAECTIFKDASGKPVPYCYDTNVLE
GSVAYESLRPDTRYVLMDGSIIQFPNTYLEGSVRVVTTFDSEYCRHGTCERSEAGVCVSTSGRWVLNNDYYRSLPGVFCGVDAVNLLTNMFTPLIQPIGA
LDISASIVAGGIVAIVVTCLAYYFMRFRRAFGEYSHVVAFNTLLFLMSFTVLCLTPVYSFLPGVYSVIYLYLTFYLTNDVSFLAHIQWMVMFTPLVPFWI
TIAYIICISTKHFYWFFSNYLKRRVVFNGVSFSTFEEAALCTFLLNKEMYLKLRSDVLLPLTQYNRYLALYNKYKYFSGAMDTTSYREAACCHLAKALND
FSNSGSDVLYQPPQTSITSAVLQ*
RGGSYTNDKACPLIAAVITREVGFVVPGLPGTILRTTNGDFLHFLPRVFSAVGNICYTPSKLIEYTDFATSACVLAAECTIFKDASGKPVPYCYDTNVLE
GSVAYESLRPDTRYVLMDGSIIQFPNTYLEGSVRVVTTFDSEYCRHGTCERSEAGVCVSTSGRWVLNNDYYRSLPGVFCGVDAVNLLTNMFTPLIQPIGA
LDISASIVAGGIVAIVVTCLAYYFMRFRRAFGEYSHVVAFNTLLFLMSFTVLCLTPVYSFLPGVYSVIYLYLTFYLTNDVSFLAHIQWMVMFTPLVPFWI
TIAYIICISTKHFYWFFSNYLKRRVVFNGVSFSTFEEAALCTFLLNKEMYLKLRSDVLLPLTQYNRYLALYNKYKYFSGAMDTTSYREAACCHLAKALND
FSNSGSDVLYQPPQTSITSAVLQ*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Codon optimised for bacterial expression.
100042 R 1000 1001 1047 19007
Full Snapgene Map