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Plasmid
pGEX6P1 SARS-CoV2 (2019-nCoV) Nsp6 (extra sequence at N-ter)
Parent Plasmid
pGEX6P1
Expressed
GST SARS-CoV2 (2019-nCoV) Nsp6 (extra sequence at N-ter)
DU Number
DU67762
Genbank
MN908947.3
Tag
GST
Species
SARS-CoV-2
Sequence of Insert
View Sequence
GGATCCGTGACCTTTCAGAGCGCGGTGAAACGCACCATTAAAGGCACCCATCATTGGCTGCTGCTGACCATTCTGACCAGCCTGCTGGTGCTGGTGCAGAGCACCCAGTGGAGCCTGTTTTTTTTTCTGTATGAAAACGCGTTTCTGCCGTTTGCGATGGGCATTATTGCGATGAGCGCGTTTGCGATGATGTTTGTGAAACATAAACATGCGTTTCTGTGCCTGTTTCTGCTGCCGAGCCTGGCGACCGTGGCGTATTTTAACATGGTGTATATGCCGGCGAGCTGGGTGATGCGCATTATGACCTGGCTGGATATGGTGGATACCAGCCTGAGCGGCTTTAAACTGAAAGATTGCGTGATGTATGCGAGCGCGGTGGTGCTGCTGATTCTGATGACCGCGCGCACCGTGTATGATGATGGCGCGCGCCGCGTGTGGACCCTGATGAACGTGCTGACCCTGGTGTATAAAGTGTATTATGGCAACGCGCTGGATCAGGCGATTAGCATGTGGGCGCTGATTATTAGCGTGACCAGCAACTATAGCGGCGTGGTGACCACCGTGATGTTTCTGGCGCGCGGCATTGTGTTTATGTGCGTGGAATATTGCCCGATTTTTTTTATTACCGGCAACACCCTGCAGTGCATTATGCTGGTGTATTGCTTTCTGGGCTATTTTTGCACCTGCTATTTTGGCCTGTTTTGCCTGCTGAACCGCTATTTTCGCCTGACCCTGGGCGTGTATGATTATCTGGTGAGCACCCAGGAATTTCGCTATATGAACAGCCAGGGCCTGCTGCCGCCGAAAAACAGCATTGATGCGTTTAAACTGAACATTAAACTGCTGGGCGTGGGCGGCAAACCGTGCATTAAAGTGGCGACCGTGCAGtgaGCGGCCGC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSVTFQSAVKRTIKGTHHWLLLTILTSLLVLVQSTQWSLFFFLYENAFLPFAMGIIAMSAFAMMFVKHKHAFLCLFLLPSLATVAYFNMVYMPASWVMRIMTWLDMVDTSLSGFKLKDCVMYASAVVLLILMTARTVYDDGARRVWTLMNVLTLVYKVYYGNALDQAISMWALIISVTSNYSGVVTTVMFLARGIVFMCVEYCPIFFITGNTLQCIMLVYCFLGYFCTCYFGLFCLLNRYFRLTLGVYDYLVSTQEFRYMNSQGLLPPKNSIDAFKLNIKLLGVGGKPCIKVATVQ*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Codon optimised for bacterial expression. Remake of DU66415 (No expression from DU66415, despite sequencing of insert looking good - remake to check no problem with backbone)
100042 R 1000 1001 1047 19007
Full Snapgene Map