This reagent and more can be found at our sister site MRC PPU Reagents & Services 
Request Now
Plasmid
pcDNA5 FRT/TO HA SARS CoV-2 Nsp12
Parent Plasmid
pcDNA5 FRT/TO HA
Expressed
HA SARS-CoV-2 Nsp12
DU Number
DU67894
Genbank
MN908947.3
Tag
HA
Species
SARS-CoV-2
Sequence of Insert
View Sequence
ggatccAACCGCGTGTGCGGCGTGAGCGCGGCGCGCCTGACCCCGTGCGGCACCGGCACCAGCACCGATGTGGTGTATCGCGCGTTTGATATTTATAACGATAAAGTGGCGGGCTTTGCGAAATTTCTGAAAACCAACTGCTGCCGCTTTCAGGAAAAAGATGAAGATGATAACCTGATTGATAGCTATTTTGTGGTGAAACGCCATACCTTTAGCAACTATCAGCATGAAGAAACCATTTATAACCTGCTGAAAGATTGCCCGGCGGTGGCGAAACATGATTTTTTTAAATTTCGCATTGATGGCGATATGGTGCCGCATATTAGCCGCCAGCGCCTGACCAAATATACCATGGCGGATCTGGTGTATGCGCTGCGCCATTTTGATGAAGGCAACTGCGATACCCTGAAAGAAATTCTGGTGACCTATAACTGCTGCGATGATGATTATTTTAACAAAAAAGATTGGTATGATTTTGTGGAAAACCCGGATATTCTGCGCGTGTATGCGAACCTGGGCGAACGCGTGCGCCAGGCGCTGCTGAAAACCGTGCAGTTTTGCGATGCGATGCGCAACGCGGGCATTGTGGGCGTGCTGACCCTGGATAACCAGGATCTGAACGGCAACTGGTATGATTTTGGCGATTTTATTCAGACCACCCCGGGCAGCGGCGTGCCGGTGGTGGATAGCTATTATAGCCTGCTGATGCCGATTCTGACCCTGACCCGCGCGCTGACCGCGGAAAGCCATGTGGATACCGATCTGACCAAACCGTATATTAAATGGGATCTGCTGAAATATGATTTTACCGAAGAACGCCTGAAACTGTTTGATCGCTATTTTAAATATTGGGATCAGACCTATCATCCGAACTGCGTGAACTGCCTGGATGATCGCTGCATTCTGCATTGCGCGAACTTTAACGTGCTGTTTAGCACCGTGTTTCCGCCGACCAGCTTTGGCCCGCTGGTGCGCAAAATTTTTGTGGATGGCGTGCCGTTTGTGGTGAGCACCGGCTATCATTTTCGCGAACTGGGCGTGGTGCATAACCAGGATGTGAACCTGCATAGCAGCCGCCTGAGCTTTAAAGAACTGCTGGTGTATGCGGCGGAcCCGGCGATGCATGCGGCGAGCGGCAACCTGCTGCTGGATAAACGCACCACCTGCTTTAGCGTGGCGGCGCTGACCAACAACGTGGCGTTTCAGACCGTGAAACCGGGCAACTTTAACAAAGATTTTTATGATTTTGCGGTGAGCAAAGGCTTTTTTAAAGAAGGCAGCAGCGTGGAACTGAAACATTTTTTTTTTGCGCAGGATGGCAACGCGGCGATTAGCGATTATGATTATTATCGCTATAACCTGCCGACCATGTGCGATATTCGCCAGCTGCTGTTTGTGGTGGAAGTGGTGGATAAATATTTTGATTGCTATGATGGCGGCTGCATTAACGCGAACCAGGTGATTGTGAACAACCTGGATAAAAGCGCGGGCTTTCCGTTTAACAAATGGGGCAAAGCGCGCCTGTATTATGATAGCATGAGCTATGAAGATCAGGATGCGCTGTTTGCGTATACCAAACGCAACGTGATTCCGACCATTACCCAGATGAACCTGAAATATGCGATTAGCGCGAAAAACCGCGCGCGCACCGTGGCGGGCGTGAGCATTTGCAGCACCATGACCAACCGCCAGTTTCATCAGAAACTGCTGAAAAGCATTGCGGCGACCCGCGGCGCGACCGTGGTGATTGGCACCAGCAAATTTTATGGCGGCTGGCATAACATGCTGAAAACCGTGTATAGCGATGTGGAAAACCCGCATCTGATGGGCTGGGATTATCCGAAATGCGATCGCGCGATGCCGAACATGCTGCGCATTATGGCGAGCCTGGTGCTGGCGCGCAAACATACCACCTGCTGCAGCCTGAGCCATCGCTTTTATCGCCTGGCGAACGAATGCGCGCAGGTGCTGAGCGAAATGGTGATGTGCGGCGGCAGCCTGTATGTGAAACCGGGCGGCACCAGCAGCGGCGATGCGACCACCGCGTATGCGAACAGCGTGTTTAACATTTGCCAGGCGGTGACCGCGAACGTGAACGCGCTGCTGAGCACCGATGGCAACAAAATTGCGGATAAATATGTGCGCAACCTGCAGCATCGCCTGTATGAATGCCTGTATCGCAACCGCGATGTGGATACCGATTTTGTGAACGAATTTTATGCGTATCTGCGCAAACATTTTAGCATGATGATTCTGAGCGATGATGCGGTGGTGTGCTTTAACAGCACCTATGCGAGCCAGGGCCTGGTGGCGAGCATTAAAAACTTTAAAAGCGTGCTGTATTATCAGAACAACGTGTTTATGAGCGAAGCGAAATGCTGGACCGAAACCGATCTGACCAAAGGCCCGCATGAATTTTGCAGCCAGCATACCATGCTGGTGAAACAGGGCGATGATTATGTGTATCTGCCGTATCCGGAcCCGAGCCGCATTCTGGGCGCGGGCTGCTTTGTGGATGATATTGTGAAAACCGATGGCACCCTGATGATTGAACGCTTTGTGAGCCTGGCGATTGATGCGTATCCGCTGACCAAACATCCGAACCAGGAATATGCGGATGTGTTTCATCTGTATCTGCAGTATATTCGCAAACTGCATGATGAACTGACCGGCCATATGCTGGATATGTATAGCGTGATGCTGACCAACGATAACACCAGCCGCTATTGGGAACCGGAATTTTATGAAGCGATGTATACCCCGCATACCGTGCTGCAGtgagcggccgc
Amino Acid Sequence
View Sequence
MYPYDVPDYAGSNRVCGVSAARLTPCGTGTSTDVVYRAFDIYNDKVAGFAKFLKTNCCRFQEKDEDDNLIDSYFVVKRHTFSNYQHEETIYNLLKDCPAV
AKHDFFKFRIDGDMVPHISRQRLTKYTMADLVYALRHFDEGNCDTLKEILVTYNCCDDDYFNKKDWYDFVENPDILRVYANLGERVRQALLKTVQFCDAM
RNAGIVGVLTLDNQDLNGNWYDFGDFIQTTPGSGVPVVDSYYSLLMPILTLTRALTAESHVDTDLTKPYIKWDLLKYDFTEERLKLFDRYFKYWDQTYHP
NCVNCLDDRCILHCANFNVLFSTVFPPTSFGPLVRKIFVDGVPFVVSTGYHFRELGVVHNQDVNLHSSRLSFKELLVYAADPAMHAASGNLLLDKRTTCF
SVAALTNNVAFQTVKPGNFNKDFYDFAVSKGFFKEGSSVELKHFFFAQDGNAAISDYDYYRYNLPTMCDIRQLLFVVEVVDKYFDCYDGGCINANQVIVN
NLDKSAGFPFNKWGKARLYYDSMSYEDQDALFAYTKRNVIPTITQMNLKYAISAKNRARTVAGVSICSTMTNRQFHQKLLKSIAATRGATVVIGTSKFYG
GWHNMLKTVYSDVENPHLMGWDYPKCDRAMPNMLRIMASLVLARKHTTCCSLSHRFYRLANECAQVLSEMVMCGGSLYVKPGGTSSGDATTAYANSVFNI
CQAVTANVNALLSTDGNKIADKYVRNLQHRLYECLYRNRDVDTDFVNEFYAYLRKHFSMMILSDDAVVCFNSTYASQGLVASIKNFKSVLYYQNNVFMSE
AKCWTETDLTKGPHEFCSQHTMLVKQGDDYVYLPYPDPSRILGAGCFVDDIVKTDGTLMIERFVSLAIDAYPLTKHPNQEYADVFHLYLQYIRKLHDELT
GHMLDMYSVMLTNDNTSRYWEPEFYEAMYTPHTVLQ*
AKHDFFKFRIDGDMVPHISRQRLTKYTMADLVYALRHFDEGNCDTLKEILVTYNCCDDDYFNKKDWYDFVENPDILRVYANLGERVRQALLKTVQFCDAM
RNAGIVGVLTLDNQDLNGNWYDFGDFIQTTPGSGVPVVDSYYSLLMPILTLTRALTAESHVDTDLTKPYIKWDLLKYDFTEERLKLFDRYFKYWDQTYHP
NCVNCLDDRCILHCANFNVLFSTVFPPTSFGPLVRKIFVDGVPFVVSTGYHFRELGVVHNQDVNLHSSRLSFKELLVYAADPAMHAASGNLLLDKRTTCF
SVAALTNNVAFQTVKPGNFNKDFYDFAVSKGFFKEGSSVELKHFFFAQDGNAAISDYDYYRYNLPTMCDIRQLLFVVEVVDKYFDCYDGGCINANQVIVN
NLDKSAGFPFNKWGKARLYYDSMSYEDQDALFAYTKRNVIPTITQMNLKYAISAKNRARTVAGVSICSTMTNRQFHQKLLKSIAATRGATVVIGTSKFYG
GWHNMLKTVYSDVENPHLMGWDYPKCDRAMPNMLRIMASLVLARKHTTCCSLSHRFYRLANECAQVLSEMVMCGGSLYVKPGGTSSGDATTAYANSVFNI
CQAVTANVNALLSTDGNKIADKYVRNLQHRLYECLYRNRDVDTDFVNEFYAYLRKHFSMMILSDDAVVCFNSTYASQGLVASIKNFKSVLYYQNNVFMSE
AKCWTETDLTKGPHEFCSQHTMLVKQGDDYVYLPYPDPSRILGAGCFVDDIVKTDGTLMIERFVSLAIDAYPLTKHPNQEYADVFHLYLQYIRKLHDELT
GHMLDMYSVMLTNDNTSRYWEPEFYEAMYTPHTVLQ*
Vector Type
Mammalian
Antibiotic
Amp
Comments
Codon optimised for bacterial expression.
100042 R 1000 1001 1047 19007
Full Snapgene Map