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Plasmid
pET15D 8His GST 3C Nsp1-Nsp2
Parent Plasmid
pET15D 8His GST
Expressed
8His-GST-3C-Nsp1-Nsp2
DU Number
DU70079
Genbank
QHD43415.1
Tag
GST
6His
Species
SARS-CoV-2
Sequence of Insert
View Sequence
GGATCCATGGAAAGCCTGGTGCCGGGCTTTAACGAAAAAACCCATGTGCAGCTGAGCCTGCCGGTGCTGCAGGTGCGCGATGTGCTGGTGCGCGGCTTTGGCGATAGCGTGGAAGAAGTGCTGAGCGAAGCGCGCCAGCATCTGAAAGATGGCACCTGCGGCCTGGTGGAAGTGGAAAAAGGCGTGCTGCCGCAGCTGGAACAGCCGTATGTGTTTATTAAACGCAGCGATGCGCGCACCGCGCCGCATGGCCATGTGATGGTGGAACTGGTGGCGGAACTGGAAGGCATTCAGTATGGCCGCAGCGGCGAAACCCTGGGCGTGCTGGTGCCGCATGTGGGCGAAATTCCGGTGGCGTATCGCAAAGTGCTGCTGCGCAAAAACGGCAACAAAGGCGCGGGCGGCCATAGCTATGGCGCGGATCTGAAAAGCTTTGATCTGGGCGATGAACTGGGCACCGATCCGTATGAAGATTTTCAGGAAAACTGGAACACCAAACATAGCAGCGGCGTGACCCGCGAACTGATGCGCGAACTGAACGGCGGCGCGTATACCCGCTATGTGGATAACAACTTTTGCGGCCCGGATGGCTATCCGCTGGAATGCATTAAAGATCTGCTGGCGCGCGCGGGCAAAGCGAGCTGCACCCTGAGCGAACAGCTGGATTTTATTGATACCAAACGCGGCGTGTATTGCTGCCGCGAACATGAACATGAAATTGCGTGGTATACCGAACGCAGCGAAAAAAGCTATGAACTGCAGACCCCGTTTGAAATTAAACTGGCGAAAAAATTTGATACCTTTAACGGCGAATGCCCGAACTTTGTGTTTCCGCTGAACAGCATTATTAAAACCATTCAGCCGCGCGTGGAAAAAAAAAAACTGGATGGCTTTATGGGCCGCATTCGCAGCGTGTATCCGGTGGCGAGCCCGAACGAATGCAACCAGATGTGCCTGAGCACCCTGATGAAATGCGATCATTGCGGCGAAACCAGCTGGCAGACCGGCGATTTTGTGAAAGCGACCTGCGAATTTTGCGGCACCGAAAACCTGACCAAAGAAGGCGCGACCACCTGCGGCTATCTGCCGCAGAACGCGGTGGTGAAAATTTATTGCCCGGCGTGCCATAACAGCGAAGTGGGCCCGGAACATAGCCTGGCGGAATATCATAACGAAAGCGGCCTGAAAACCATTCTGCGCAAAGGtGGaCGCACCATTGCGTTTGGCGGCTGCGTGTTTAGCTATGTGGGCTGCCATAACAAATGCGCGTATTGGGTGCCGCGCGCGAGCGCGAACATTGGCTGCAACCATACCGGCGTGGTGGGCGAAGGCAGCGAAGGCCTGAACGATAACCTGCTGGAAATTCTGCAGAAAGAAAAAGTGAACATTAACATTGTGGGCGATTTTAAACTGAACGAAGAAATTGCGATTATTCTGGCGAGCTTTAGCGCGAGCACCAGCGCGTTTGTGGAAACCGTGAAAGGCCTGGATTATAAAGCGTTTAAACAGATTGTGGAAAGCTGCGGCAACTTTAAAGTGACCAAAGGCAAAGCGAAAAAAGGCGCGTGGAACATTGGCGAACAGAAAAGCATTCTGAGCCCGCTGTATGCGTTTGCGAGCGAAGCGGCGCGCGTGGTGCGCAGCATTTTTAGCCGCACCCTGGAAACCGCGCAGAACAGCGTGCGCGTGCTGCAGAAAGCGGCGATTACCATTCTGGATGGCATTAGCCAGTATAGCCTGCGCCTGATTGATGCGATGATGTTTACCAGCGATCTGGCGACCAACAACCTGGTGGTGATGGCGTATATTACCGGCGGCGTGGTGCAGCTGACCAGCCAGTGGCTGACCAACATTTTTGGCACCGTGTATGAAAAACTGAAACCGGTGCTGGATTGGCTGGAAGAAAAATTTAAAGAAGGCGTGGAATTTCTGCGCGATGGCTGGGAAATTGTGAAATTTATTAGCACCTGCGCGTGCGAAATTGTGGGCGGCCAGATTGTGACCTGCGCGAAAGAAATTAAAGAAAGCGTGCAGACCTTTTTTAAACTGGTGAACAAATTTCTGGCGCTGTGCGCGGATAGCATTATTATTGGCGGCGCGAAACTGAAAGCGCTGAACCTGGGCGAAACCTTTGTGACCCATAGCAAAGGCCTGTATCGCAAATGCGTGAAAAGCCGCGAAGAAACCGGCCTGCTGATGCCGCTGAAAGCGCCGAAAGAAATTATTTTTCTGGAAGGCGAAACCCTGCCGACCGAAGTGCTGACCGAAGAAGTGGTGCTGAAAACCGGCGATCTGCAGCCGCTGGAACAGCCGACCAGCGAAGCGGTGGAAGCGCCGCTGGTGGGCACCCCGGTGTGCATTAACGGCCTGATGCTGCTGGAAATTAAAGATACCGAAAAATATTGCGCGCTGGCGCCGAACATGATGGTGACCAACAACACCTTTACCCTGAAAGGCGGCtgaGCGGCCGC
Amino Acid Sequence
View Sequence
MGHHHHHHHHSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAE
ISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDK
YLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMESLVPGFNEKTHVQLSLPVLQVRDVLVRGFGDSVEEVLSEARQHLKDGTCGLVEVEKGV
LPQLEQPYVFIKRSDARTAPHGHVMVELVAELEGIQYGRSGETLGVLVPHVGEIPVAYRKVLLRKNGNKGAGGHSYGADLKSFDLGDELGTDPYEDFQEN
WNTKHSSGVTRELMRELNGGAYTRYVDNNFCGPDGYPLECIKDLLARAGKASCTLSEQLDFIDTKRGVYCCREHEHEIAWYTERSEKSYELQTPFEIKLA
KKFDTFNGECPNFVFPLNSIIKTIQPRVEKKKLDGFMGRIRSVYPVASPNECNQMCLSTLMKCDHCGETSWQTGDFVKATCEFCGTENLTKEGATTCGYL
PQNAVVKIYCPACHNSEVGPEHSLAEYHNESGLKTILRKGGRTIAFGGCVFSYVGCHNKCAYWVPRASANIGCNHTGVVGEGSEGLNDNLLEILQKEKVN
INIVGDFKLNEEIAIILASFSASTSAFVETVKGLDYKAFKQIVESCGNFKVTKGKAKKGAWNIGEQKSILSPLYAFASEAARVVRSIFSRTLETAQNSVR
VLQKAAITILDGISQYSLRLIDAMMFTSDLATNNLVVMAYITGGVVQLTSQWLTNIFGTVYEKLKPVLDWLEEKFKEGVEFLRDGWEIVKFISTCACEIV
GGQIVTCAKEIKESVQTFFKLVNKFLALCADSIIIGGAKLKALNLGETFVTHSKGLYRKCVKSREETGLLMPLKAPKEIIFLEGETLPTEVLTEEVVLKT
GDLQPLEQPTSEAVEAPLVGTPVCINGLMLLEIKDTEKYCALAPNMMVTNNTFTLKGG*
ISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDK
YLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMESLVPGFNEKTHVQLSLPVLQVRDVLVRGFGDSVEEVLSEARQHLKDGTCGLVEVEKGV
LPQLEQPYVFIKRSDARTAPHGHVMVELVAELEGIQYGRSGETLGVLVPHVGEIPVAYRKVLLRKNGNKGAGGHSYGADLKSFDLGDELGTDPYEDFQEN
WNTKHSSGVTRELMRELNGGAYTRYVDNNFCGPDGYPLECIKDLLARAGKASCTLSEQLDFIDTKRGVYCCREHEHEIAWYTERSEKSYELQTPFEIKLA
KKFDTFNGECPNFVFPLNSIIKTIQPRVEKKKLDGFMGRIRSVYPVASPNECNQMCLSTLMKCDHCGETSWQTGDFVKATCEFCGTENLTKEGATTCGYL
PQNAVVKIYCPACHNSEVGPEHSLAEYHNESGLKTILRKGGRTIAFGGCVFSYVGCHNKCAYWVPRASANIGCNHTGVVGEGSEGLNDNLLEILQKEKVN
INIVGDFKLNEEIAIILASFSASTSAFVETVKGLDYKAFKQIVESCGNFKVTKGKAKKGAWNIGEQKSILSPLYAFASEAARVVRSIFSRTLETAQNSVR
VLQKAAITILDGISQYSLRLIDAMMFTSDLATNNLVVMAYITGGVVQLTSQWLTNIFGTVYEKLKPVLDWLEEKFKEGVEFLRDGWEIVKFISTCACEIV
GGQIVTCAKEIKESVQTFFKLVNKFLALCADSIIIGGAKLKALNLGETFVTHSKGLYRKCVKSREETGLLMPLKAPKEIIFLEGETLPTEVLTEEVVLKT
GDLQPLEQPTSEAVEAPLVGTPVCINGLMLLEIKDTEKYCALAPNMMVTNNTFTLKGG*
Vector Type
Bacterial
Antibiotic
Amp
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