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Ubiquitin

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Plasmid
pET24-Ubiquitin (silent a220c)
Parent Plasmid
pET24a original
Expressed
untagged Ubiquitin
DU Number
DU20027
Genbank
P62988*
Tag
untagged
Species
Human
Sequence of Insert
CATATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGA
AGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACA
AGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAG
CTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCAC
CCTGCACCTGGTCCTCCGTCTCCGAGGTGGGTGATAAGGATCC
Amino Acid Sequence
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQL
EDGRTLSDYNIQKESTLHLVLRLRGG
Vector Type
Bacterial
Antibiotic
Kan
Comments
Bacterial expression construct for untagged ubiquitin (Arg73 codon AGA mutated to more common CGA)

Differs from reference sequence in having silent mutation at Arg73 (a220c substitution conferred by reverse primer 54389).
T7 tag in pET24a removed by subcloning into the Nde1 site, to give untagged protein.

Estimated MW of expressed protein : 8565 Daltons
100042 R 1000 1001 1047 19007
Full Snapgene Map
20027.dna