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Plasmid
pGEX6P1 SARS CoV HSZ-Cc S protein (Custom)
Parent Plasmid
pGEX6P1
Expressed
GST 3C SARS CoV HSZ-Cc S protein
DU Number
DU67727
Genbank
AY394995.1
Tag
GST
Species
SARS-CoV
Sequence of Insert
View Sequence
GGATCCATGTTTATTTTTCTGCTGTTTCTGACCCTGACCAGCGGCAGCGATCTGGAcCGCTGCACCACCTTTGATGATGTGCAGGCGCCGAACTATACCCAGCATACCAGCAGCATGCGCGGCGTGTATTATCCGGATGAAATTTTTCGCAGCGATACCCTGTATCTGACCCAGGAcCTGTTTCTGCCGTTTTATAGCAACGTGACCGGCTTTCATACCATTAACCATACCTTTGATAACCCGGTGATTCCGTTTAAAGATGGCATTTATTTTGCGGCGACCGAAAAAAGCAACGTGGTGCGCGGCTGGGTGTTTGGCAGCACCATGAACAACAAAAGCCAGAGCGTGATTATTATTAACAACAGCACCAACGTGGTGATTCGCGCGTGCAACTTTGAACTGTGCGATAACCCGTTTTTTGCGGTGAGCAAACCGATGGGCACCCAGACCCATACCATGATTTTTGATAACGCGTTTAACTGCACCTTTGAATATATTAGCGATGCGTTTAGCCTGGATGTGAGCGAAAAAAGCGGCAACTTTAAACATCTGCGCGAATTTGTGTTTAAAAACAAAGATGGCTTTCTGTATGTGTATAAAGGCTATCAGCCGATTGATGTGGTGCGCGATCTGCCGAGCGGCTTTAACACCCTGAAACCGATTTTTAAACTGCCGCTGGGCATTAACATTACCAACTTTCGCGCGATTCTGACCGCGTTTAGCCCGGCGCAGGATACCTGGGGCACCAGCGCGGCGGCGTATTTTGTGGGCTATCTGAAACCGACCACCTTTATGCTGAAATATGATGAAAACGGCACCATTACCGATGCGGTGGATTGCAGCCAGAACCCGCTGGCGGAACTGAAATGCAGCGTGAAAAGCTTTGAAATTGATAAAGGCATTTATCAGACCAGCAACTTTCGCGTGGTGCCGAGCGGCGATGTGGTGCGCTTTCCGAACATTACCAACCTGTGCCCGTTTGGCGAAGTGTTTAACGCGACCAAATTTCCGAGCGTGTATGCGTGGGAACGCAAAAAAATTAGCAACTGCGTGGCGGATTATAGCGTGCTGTATAACAGCACCTTTTTTAGCACCTTTAAATGCTATGGCGTGAGCGCGACCAAACTGAACGATCTGTGCTTTAGCAACGTGTATGCGGATAGCTTTGTGGTGAAAGGCGATGATGTGCGCCAGATTGCGCCGGGCCAGACCGGCGTGATTGCGGATTATAACTATAAACTGCCGGATGATTTTATGGGCTGCGTGCTGGCGTGGAACACCCGCAACATTGATGCGACCAGCACCGGCAACTATAACTATAAATATCGCTATCTGCGCCATGGCAAACTGCGCCCGTTTGAACGCGATATTAGCAACGTGCCGTTTAGCCCGGATGGCAAACCGTGCACCCCGCCGGCGCTGAACTGCTATTGGCCGCTGAACGATTATGGCTTTTATACCACCACCGGCATTGGCTATCAGCCGTATCGCGTGGTGGTGCTGAGCTTTGAACTGCTGAACGCGCCGGCGACCGTGTGCGGCCCGAAACTGAGCACCGATCTGATTAAAAACCAGTGCGTGAACTTTAACTTTAACGGCCTGACCGGCACCGGCGTGCTGACCCCGAGCAGCAAACGCTTTCAGCCGTTTCAGCAGTTTGGCCGCGATGTGAGCGATTTTACCGATAGCGTGCGCGATCCGAAAACCAGCGAAATTCTGGATATTAGCCCGTGCAGCTTTGGCGGCGTGAGCGTGATTACCCCGGGCACCAACGCGAGCAGCGAAGTGGCGGTGCTGTATCAGGATGTGAACTGCACCGATGTGAGCACCGCGATTCATGCGGATCAGCTGACCCCGGCGTGGCGCATTTATAGCACCGGCAACAACGTGTTTCAGACCCAGGCGGGCTGCCTGATTGGCGCGGAACATGTGGATACCAGCTATGAATGCGATATTCCGATTGGCGCGGGCATTTGCGCGAGCTATCATACCGTGAGCCTGCTGCGCAGCACCAGCCAGAAAAGCATTGTGGCGTATACCATGAGCCTGGGCGCGGATAGCAGCATTGCGTATAGCAACAACACCATTGCGATTCCGACCAACTTTAGCATTAGCATTACCACCGAAGTGATGCCGGTGAGCATGGCGAAAACCAGCGTGGATTGCAACATGTATATTTGCGGCGATAGCACCGAATGCGCGAACCTGCTGCTGCAGTATGGCAGCTTTTGCACCCAGCTGAACCGCGCGCTGAGCGGCATTGCGGCGGAACAGGATCGCAACACCCGCGAAGTGTTTGCGCAGGTGAAACAGATGTATAAAACCCCGACCCTGAAAGATTTTGGCGGCTTTAACTTTAGCCAGATTCTGCCGGAcCCGCTGAAACCGACCAAACGCAGCTTTATTGAAGATCTGCTGTTTAACAAAGTGACCCTGGCGGATGCGGGCTTTATGAAACAGTATGGCGAATGCCTGGGCGATATTAACGCGCGCGATCTGATTTGCGCGCAGAAATTTAACGGCCTGACCGTGCTGCCGCCGCTGCTGACCGATGATATGATTGCGGCGTATACCGCGGCGCTGGTGAGCGGCACCGCGACCGCGGGCTGGACCTTTGGCGCGGGCGCGGCGCTGCAGATTCCGTTTGCGATGCAGATGGCGTATCGCTTTAACGGCATTGGCGTGACCCAGAACGTGCTGTATGAAAACCAGAAACAGATTGCGAACCAGTTTAACAAAGCGATTAGCCAGATTCAGGAAAGCCTGACCACCACCAGCACCGCGCTGGGCAAACTGCAGGATGTGGTGAACCAGAACGCGCAGGCGCTGAACACCCTGGTGAAACAGCTGAGCAGCAACTTTGGCGCGATTAGCAGCGTGCTGAACGATATTCTGAGCCGCCTGGATAAAGTGGAAGCGGAAGTGCAGATTGATCGCCTGATTACCGGCCGCCTGCAGAGCCTGCAGACCTATGTGACCCAGCAGCTGATTCGCGCGGCGGAAATTCGCGCGAGCGCGAACCTGGCGGCGACCAAAATGAGCGAATGCGTGCTGGGCCAGAGCAAACGCGTGGATTTTTGCGGCAAAGGCTATCATCTGATGAGCTTTCCGCAGGCGGCGCCGCATGGCGTGGTGTTTCTGCATGTGACCTATGTGCCGAGCCAGGAACGCAACTTTACCACCGCGCCGGCGATTTGCCATGAAGGCAAAGCGTATTTTCCGCGCGAAGGCGTGTTTGTGTTTAACGGCACCAGCTGGTTTATTACCCAGCGCAACTTTTTTAGCCCGCAGATTATTACCACCGATAACACCTTTGTGAGCGGCAACTGCGATGTGGTGATTGGCATTATTAACAACACCGTGTATGATCCGCTGCAGCCGGAACTGGATAGCTTTAAAGAAGAACTGGATAAATATTTTAAAAACCATACCAGCCCGGATGTGGATCTGGGCGATATTAGCGGCATTAACGCGAGCGTGGTGAACATTCAGGAAGAAATTGATCGCCTGAACGAAGTGGCGAAAAACCTGAACGAAAGCCTGATTGATCTGCAGGAACTGGGCAAATATGAACAGTATATTAAATGGCCGTGGTATGTGTGGCTGGGCTTTATTGCGGGCCTGATTGCGATTGTGATGGTGACCATTCTGCTGTGCTGCATGACCAGCTGCTGCAGCTGCCTGAAAGGCGCGTGCAGCTGCGGCAGCTGCTGCAAATTTGATGAAGATGATAGCGAACCGGTGTTTAAAGGCGTGAAACTGCATTATACCtgaGCGGCCGC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMFIFLLFLTLTSGSDLDRCTTFDDVQAPNYTQHTSSMRGVYYPDEIFRSDTLYLTQDLFLPFYSNVTGFHTINHTFDNPVIPFKDGIYFAATEKSNVVRGWVFGSTMNNKSQSVIIINNSTNVVIRACNFELCDNPFFAVSKPMGTQTHTMIFDNAFNCTFEYISDAFSLDVSEKSGNFKHLREFVFKNKDGFLYVYKGYQPIDVVRDLPSGFNTLKPIFKLPLGINITNFRAILTAFSPAQDTWGTSAAAYFVGYLKPTTFMLKYDENGTITDAVDCSQNPLAELKCSVKSFEIDKGIYQTSNFRVVPSGDVVRFPNITNLCPFGEVFNATKFPSVYAWERKKISNCVADYSVLYNSTFFSTFKCYGVSATKLNDLCFSNVYADSFVVKGDDVRQIAPGQTGVIADYNYKLPDDFMGCVLAWNTRNIDATSTGNYNYKYRYLRHGKLRPFERDISNVPFSPDGKPCTPPALNCYWPLNDYGFYTTTGIGYQPYRVVVLSFELLNAPATVCGPKLSTDLIKNQCVNFNFNGLTGTGVLTPSSKRFQPFQQFGRDVSDFTDSVRDPKTSEILDISPCSFGGVSVITPGTNASSEVAVLYQDVNCTDVSTAIHADQLTPAWRIYSTGNNVFQTQAGCLIGAEHVDTSYECDIPIGAGICASYHTVSLLRSTSQKSIVAYTMSLGADSSIAYSNNTIAIPTNFSISITTEVMPVSMAKTSVDCNMYICGDSTECANLLLQYGSFCTQLNRALSGIAAEQDRNTREVFAQVKQMYKTPTLKDFGGFNFSQILPDPLKPTKRSFIEDLLFNKVTLADAGFMKQYGECLGDINARDLICAQKFNGLTVLPPLLTDDMIAAYTAALVSGTATAGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKQIANQFNKAISQIQESLTTTSTALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQAAPHGVVFLHVTYVPSQERNFTTAPAICHEGKAYFPREGVFVFNGTSWFITQRNFFSPQIITTDNTFVSGNCDVVIGIINNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQEEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYVWLGFIAGLIAIVMVTILLCCMTSCCSCLKGACSCGSCCKFDEDDSEPVFKGVKLHYT*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Codon optimised for bacterial expression.
100042 R 1000 1001 1047 19007
Full Snapgene Map