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Plasmid
pGEX6P1 MERS CoV S protein (Custom)
Parent Plasmid
pGEX6P1
Expressed
GST 3C MERS CoV S protein
DU Number
DU67745
Genbank
MG912608.1
Tag
GST
Species
MERS-CoV
Sequence of Insert
View Sequence
GGATCCATGATTCATAGCGTGTTTCTGCTGATGTTTCTGCTGACCCCGACCGAAAGCTATGTGGATGTGGGCCCGGATAGCGTGAAAAGCGCGTGCATTGAAGTGGATATTCAGCAGACCTTTTTTGATAAAACCTGGCCGCGCCCGATTGATGTGAGCAAAGCGGATGGCATTATTTATCCGCAGGGCCGCACCTATAGCAACATTACCATTACCTATCAGGGCCTGTTTCCGTATCAGGGCGATCATGGCGATATGTATGTGTATAGCGCGGGCCATGCGACCGGCACCACCCCGCAGAAACTGTTTGTGGCGAACTATAGCCAGGATGTGAAACAGTTTGCGAACGGCTTTGTGGTGCGCATTGGCGCGGCGGCGAACAGCACCGGCACCGTGATTATTAGCCCGAGCACCAGCGCGACCATTCGCAAAATTTATCCGGCGTTTATGCTGGGCAGCAGCGTGGGCAACTTTAGCGATGGCAAAATGGGCCGCTTTTTTAACCATACCCTGGTGCTGCTGCCGGATGGCTGCGGCACCCTGCTGCGCGCGTTTTATTGCATTCTGGAACCGCGCAGCGGCAACCATTGCCCGGCGGGCAACAGCTATACCAGCTTTGCGACCTATCATACCCCGGCGACCGATTGCAGCGATGGCAACTATAACCGCAACGCGAGCCTGAACAGCTTTAAAGAATATTTTAACCTGCGCAACTGCACCTTTATGTATACCTATAACATTACCGAAGATGAAATTCTGGAATGGTTTGGCATTACCCAGACCGCGCAGGGCGTGCATCTGTTTAGCAGCCGCTATGTGGATCTGTATGGCGGCAACATGTTTCAGTTTGCGACCCTGCCGGTGTATGATACCATTAAATATTATAGCATTATTCCGCATAGCATTCGCAGCATTCAGAGCGATCGCAAAGCGTGGGCGGCGTTTTATGTGTATAAACTGCAGCCGCTGACCTTTCTGCTGGATTTTAGCGTGGATGGCTATATTCGCCGCGCGATTGATTGCGGCTTTAACGATCTGAGCCAGCTGCATTGCAGCTATGAAAGCTTTGATGTGGAAAGCGGCGTGTATAGCGTGAGCAGCTTTGAAGCGAAACCGAGCGGCAGCGTGGTGGAACAGGCGGAAGGCGTGGAATGCGATTTTAGCCCGCTGCTGAGCGGCACCCCGCCGCAGGTGTATAACTTTAAACGCCTGGTGTTTACCAACTGCAACTATAACCTGACCAAACTGCTGAGCCTGTTTAGCGTGAACGATTTTACCTGCAGCCAGATTAGCCCGGCGGCGATTGCGAGCAACTGCTATAGCAGCCTGATTCTGGATTATTTTAGCTATCCGCTGAGCATGAAAAGCGATCTGAGCGTGAGCAGCGCGGGCCCGATTAGCCAGTTTAACTATAAACAGAGCTTTAGCAACCCGACCTGCCTGATTCTGGCGACCGTGCCGCATAACCTGACCACCATTACCAAACCGCTGAAATATAGCTATATTAACAAATGCAGCCGCCTGCTGAGCGATGATCGCACCGAAGTGCCGCAGCTGGTGAACGCGAACCAGTATAGCCCGTGCGTGAGCATTGTGCCGAGCACCGTGTGGGAAGATGGCGATTATTATCGCAAACAGCTGAGCCCGCTGGAAGGCGGCGGCTGGCTGGTGGCGAGCGGCAGCACCGTGGCGATGACCGAACAGCTGCAGATGGGCTTTGGCATTACCGTGCAGTATGGCACCGATACCAACAGCGTGTGCCCGAAACTGGAATTTGCGAACGATACCAAAATTGCGAGCCAGCTGGGCAACTGCGTGGAATATAGCCTGTATGGCGTGAGCGGtagaGGCGTGTTTCAGAACTGCACCGCGGTGGGCGTGCGCCAGCAGCGCTTTGTGTATGATGCGTATCAGAACCTGGTGGGCTATTATAGCGATGATGGCAACTATTATTGCCTGCGCGCGTGCGTGAGCGTGCCGGTGAGCGTGATTTATGATAAAGAAACCAAAACCCATGCGACCCTGTTTGGCAGCGTGGCGTGCGAACATATTAGCAGCACCATGAGCCAGTATAGCCGCAGCACCCGCAGCATGCTGAAACGCCGCGATAGCACCTATGGCCCGCTGCAGACCCCGGTGGGCTGCGTGCTGGGCCTGGTGAACAGCAGCCTGTTTGTGGAAGATTGCAAACTGCCGCTGGGCCAGAGCCTGTGCGCGCTGCCGGATACCCCGAGCACCCTGACCCCGCGCAGCGTGCGCAGCGTGCCGGGCGAAATGCGCCTGGCGAGCATTGCGTTTAACCATCCGATTCAGGTGGATCAGCTGAACAGCAGCTATTTTAAACTGAGCATTCCGACCAACTTTAGCTTTGGCGTGACCCAGGAATATATTCAGACCACCATTCAGAAAGTGACCGTGGATTGCAAACAGTATGTGTGCAACGGCTTTCAGAAATGCGAACAGCTGCTGCGCGAATATGGCCAGTTTTGCAGCAAAATTAACCAGGCGCTGCATGGCGCGAACCTGCGCCAGGATGATAGCGTGCGCAACCTGTTTGCGAGCGTGAAAAGCAGCCAGAGCAGCCCGATTATTCCGGGCTTTGGCGGCGATTTTAACCTGACCCTGCTGGAACCGGTGAGCATTAGCACCGGCAGCCGCAGCGCGCGCAGCGCGATTGAAGATCTGCTGTTTGATAAAGTGACCATTGCGGAcCCGGGCTATATGCAGGGCTATGATGATTGCATGCAGCAGGGCCCGGCGAGCGCGCGCGATCTGATTTGCGCGCAGTATGTGGCGGGCTATAAAGTGCTGCCGCCGCTGATGGATGTGAACATGGAAGCGGCGTATACCAGCAGCCTGCTGGGCAGCATTGCGGGCGTGGGCTGGACCGCGGGCCTGAGCAGCTTTGCGGCGATTCCGTTTGCGCAGAGCATTTTTTATCGCCTGAACGGCGTGGGCATTACCCAGCAGGTGCTGAGCGAAAACCAGAAACTGATTGCGAACAAATTTAACCAGGCGCTGGGCGCGATGCAGACCGGCTTTACCACCACCAACGAAGCGTTTCGCAAAGTGCAGGATGCGGTGAACAACAACGCGCAGGCGCTGAGCAAACTGGCGAGCGAACTGAGCAACACCTTTGGCGCGATTAGCGCGAGCATTGGCGATATTATTCAGCGCCTGGATGTGCTGGAACAGGATGCGCAGATTGATCGCCTGATTAACGGCCGCCTGACCACCCTGAACGCGTTTGTGGCGCAGCAGCTGGTGCGCAGCGAAAGCGCGGCGCTGAGCGCGCAGCTGGCGAAAGATAAAGTGAACGAATGCGTGAAAGCGCAGAGCAAACGCAGCGGCTTTTGCGGCCAGGGCACCCATATTGTGAGCTTTGTGGTGAACGCGCCGAACGGCCTGTATTTTATGCATGTGGGCTATTATCCGAGCAACCATATTGAAGTGGTGAGCGCGTATGGCCTGTGCGATGCGGCGAACCCGACCAACTGCATTGCGCCGGTGAACGGCTATTTTATTAAAACCAACAACACCCGCATTGTGGATGAATGGAGCTATACCGGCAGCAGCTTTTATGCGCCGGAACCGATTACCAGCCTGAACACCAAATATGTGGCGCCGCAGGTGACCTATCAGAACATTAGCACCAACCTGCCGCCGCCGCTGCTGGGCAACAGCACCGGCATTGATTTTCAGGATGAACTGGATGAATTTTTTAAAAACGTGAGCACCAGCATTCCGAACTTTGGCAGCCTGACCCAGATTAACACCACCCTGCTGGATCTGACCTATGAAATGCTGAGCCTGCAGCAGGTGGTGAAAGCGCTGAACGAAAGCTATATTGATCTGAAAGAACTGGGCAACTATACCTATTATAACAAATGGCCGTGGTATATTTGGCTGGGCTTTATTGCGGGCCTGGTGGCGCTGGCGCTGTGCGTGTTTTTTATTCTGTGCTGCACCGGCTGCGGCACCAACTGCATGGGCAAACTGAAATGCAACCGCTGCTGCGATCGCTATGAAGAATATGATCTGGAACCGCATAAAGTGCATGTGCATtgaGCGGCCGC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMIHSVFLLMFLLTPTESYVDVGPDSVKSACIEVDIQQTFFDKTWPRPIDVSKADGIIYPQGRTYSNITITYQGLFPYQGDHGDMYVYSAGHATGTTPQKLFVANYSQDVKQFANGFVVRIGAAANSTGTVIISPSTSATIRKIYPAFMLGSSVGNFSDGKMGRFFNHTLVLLPDGCGTLLRAFYCILEPRSGNHCPAGNSYTSFATYHTPATDCSDGNYNRNASLNSFKEYFNLRNCTFMYTYNITEDEILEWFGITQTAQGVHLFSSRYVDLYGGNMFQFATLPVYDTIKYYSIIPHSIRSIQSDRKAWAAFYVYKLQPLTFLLDFSVDGYIRRAIDCGFNDLSQLHCSYESFDVESGVYSVSSFEAKPSGSVVEQAEGVECDFSPLLSGTPPQVYNFKRLVFTNCNYNLTKLLSLFSVNDFTCSQISPAAIASNCYSSLILDYFSYPLSMKSDLSVSSAGPISQFNYKQSFSNPTCLILATVPHNLTTITKPLKYSYINKCSRLLSDDRTEVPQLVNANQYSPCVSIVPSTVWEDGDYYRKQLSPLEGGGWLVASGSTVAMTEQLQMGFGITVQYGTDTNSVCPKLEFANDTKIASQLGNCVEYSLYGVSGRGVFQNCTAVGVRQQRFVYDAYQNLVGYYSDDGNYYCLRACVSVPVSVIYDKETKTHATLFGSVACEHISSTMSQYSRSTRSMLKRRDSTYGPLQTPVGCVLGLVNSSLFVEDCKLPLGQSLCALPDTPSTLTPRSVRSVPGEMRLASIAFNHPIQVDQLNSSYFKLSIPTNFSFGVTQEYIQTTIQKVTVDCKQYVCNGFQKCEQLLREYGQFCSKINQALHGANLRQDDSVRNLFASVKSSQSSPIIPGFGGDFNLTLLEPVSISTGSRSARSAIEDLLFDKVTIADPGYMQGYDDCMQQGPASARDLICAQYVAGYKVLPPLMDVNMEAAYTSSLLGSIAGVGWTAGLSSFAAIPFAQSIFYRLNGVGITQQVLSENQKLIANKFNQALGAMQTGFTTTNEAFRKVQDAVNNNAQALSKLASELSNTFGAISASIGDIIQRLDVLEQDAQIDRLINGRLTTLNAFVAQQLVRSESAALSAQLAKDKVNECVKAQSKRSGFCGQGTHIVSFVVNAPNGLYFMHVGYYPSNHIEVVSAYGLCDAANPTNCIAPVNGYFIKTNNTRIVDEWSYTGSSFYAPEPITSLNTKYVAPQVTYQNISTNLPPPLLGNSTGIDFQDELDEFFKNVSTSIPNFGSLTQINTTLLDLTYEMLSLQQVVKALNESYIDLKELGNYTYYNKWPWYIWLGFIAGLVALALCVFFILCCTGCGTNCMGKLKCNRCCDRYEEYDLEPHKVHVH*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Codon optimised for bacterial expression.
100042 R 1000 1001 1047 19007
Full Snapgene Map