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Plasmid
pMEX3Cb SARS-CoV-2 NSP11/12
Parent Plasmid
pMEX3Cb
Expressed
MBP 3C SARS-CoV-2 NSP11/12
DU Number
DU67851
Genbank
MN908947.3
Tag
MBP
Species
SARS-CoV-2
Sequence of Insert
View Sequence
GGATCCAACCGCGTGTGCGGCGTGAGCGCGGCGCGCCTGACCCCGTGCGGCACCGGCACCAGCACCGATGTGGTGTATCGCGCGTTTGATATTTATAACGATAAAGTGGCGGGCTTTGCGAAATTTCTGAAAACCAACTGCTGCCGCTTTCAGGAAAAAGATGAAGATGATAACCTGATTGATAGCTATTTTGTGGTGAAACGCCATACCTTTAGCAACTATCAGCATGAAGAAACCATTTATAACCTGCTGAAAGATTGCCCGGCGGTGGCGAAACATGATTTTTTTAAATTTCGCATTGATGGCGATATGGTGCCGCATATTAGCCGCCAGCGCCTGACCAAATATACCATGGCGGATCTGGTGTATGCGCTGCGCCATTTTGATGAAGGCAACTGCGATACCCTGAAAGAAATTCTGGTGACCTATAACTGCTGCGATGATGATTATTTTAACAAAAAAGATTGGTATGATTTTGTGGAAAACCCGGATATTCTGCGCGTGTATGCGAACCTGGGCGAACGCGTGCGCCAGGCGCTGCTGAAAACCGTGCAGTTTTGCGATGCGATGCGCAACGCGGGCATTGTGGGCGTGCTGACCCTGGATAACCAGGATCTGAACGGCAACTGGTATGATTTTGGCGATTTTATTCAGACCACCCCGGGCAGCGGCGTGCCGGTGGTGGATAGCTATTATAGCCTGCTGATGCCGATTCTGACCCTGACCCGCGCGCTGACCGCGGAAAGCCATGTGGATACCGATCTGACCAAACCGTATATTAAATGGGATCTGCTGAAATATGATTTTACCGAAGAACGCCTGAAACTGTTTGATCGCTATTTTAAATATTGGGATCAGACCTATCATCCGAACTGCGTGAACTGCCTGGATGATCGCTGCATTCTGCATTGCGCGAACTTTAACGTGCTGTTTAGCACCGTGTTTCCGCCGACCAGCTTTGGCCCGCTGGTGCGCAAAATTTTTGTGGATGGCGTGCCGTTTGTGGTGAGCACCGGCTATCATTTTCGCGAACTGGGCGTGGTGCATAACCAGGATGTGAACCTGCATAGCAGCCGCCTGAGCTTTAAAGAACTGCTGGTGTATGCGGCGGAcCCGGCGATGCATGCGGCGAGCGGCAACCTGCTGCTGGATAAACGCACCACCTGCTTTAGCGTGGCGGCGCTGACCAACAACGTGGCGTTTCAGACCGTGAAACCGGGCAACTTTAACAAAGATTTTTATGATTTTGCGGTGAGCAAAGGCTTTTTTAAAGAAGGCAGCAGCGTGGAACTGAAACATTTTTTTTTTGCGCAGGATGGCAACGCGGCGATTAGCGATTATGATTATTATCGCTATAACCTGCCGACCATGTGCGATATTCGCCAGCTGCTGTTTGTGGTGGAAGTGGTGGATAAATATTTTGATTGCTATGATGGCGGCTGCATTAACGCGAACCAGGTGATTGTGAACAACCTGGATAAAAGCGCGGGCTTTCCGTTTAACAAATGGGGCAAAGCGCGCCTGTATTATGATAGCATGAGCTATGAAGATCAGGATGCGCTGTTTGCGTATACCAAACGCAACGTGATTCCGACCATTACCCAGATGAACCTGAAATATGCGATTAGCGCGAAAAACCGCGCGCGCACCGTGGCGGGCGTGAGCATTTGCAGCACCATGACCAACCGCCAGTTTCATCAGAAACTGCTGAAAAGCATTGCGGCGACCCGCGGCGCGACCGTGGTGATTGGCACCAGCAAATTTTATGGCGGCTGGCATAACATGCTGAAAACCGTGTATAGCGATGTGGAAAACCCGCATCTGATGGGCTGGGATTATCCGAAATGCGATCGCGCGATGCCGAACATGCTGCGCATTATGGCGAGCCTGGTGCTGGCGCGCAAACATACCACCTGCTGCAGCCTGAGCCATCGCTTTTATCGCCTGGCGAACGAATGCGCGCAGGTGCTGAGCGAAATGGTGATGTGCGGCGGCAGCCTGTATGTGAAACCGGGCGGCACCAGCAGCGGCGATGCGACCACCGCGTATGCGAACAGCGTGTTTAACATTTGCCAGGCGGTGACCGCGAACGTGAACGCGCTGCTGAGCACCGATGGCAACAAAATTGCGGATAAATATGTGCGCAACCTGCAGCATCGCCTGTATGAATGCCTGTATCGCAACCGCGATGTGGATACCGATTTTGTGAACGAATTTTATGCGTATCTGCGCAAACATTTTAGCATGATGATTCTGAGCGATGATGCGGTGGTGTGCTTTAACAGCACCTATGCGAGCCAGGGCCTGGTGGCGAGCATTAAAAACTTTAAAAGCGTGCTGTATTATCAGAACAACGTGTTTATGAGCGAAGCGAAATGCTGGACCGAAACCGATCTGACCAAAGGCCCGCATGAATTTTGCAGCCAGCATACCATGCTGGTGAAACAGGGCGATGATTATGTGTATCTGCCGTATCCGGAcCCGAGCCGCATTCTGGGCGCGGGCTGCTTTGTGGATGATATTGTGAAAACCGATGGCACCCTGATGATTGAACGCTTTGTGAGCCTGGCGATTGATGCGTATCCGCTGACCAAACATCCGAACCAGGAATATGCGGATGTGTTTCATCTGTATCTGCAGTATATTCGCAAACTGCATGATGAACTGACCGGCCATATGCTGGATATGTATAGCGTGATGCTGACCAACGATAACACCAGCCGCTATTGGGAACCGGAATTTTATGAAGCGATGTATACCCCGCATACCGTGCTGCAGtgaGCGGCCGC
Amino Acid Sequence
View Sequence
MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRY
NGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLI
KNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPL
GAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQGP
LGSNRVCGVSAARLTPCGTGTSTDVVYRAFDIYNDKVAGFAKFLKTNCCRFQEKDEDDNLIDSYFVVKRHTFSNYQHEETIYNLLKDCPAVAKHDFFKFR
IDGDMVPHISRQRLTKYTMADLVYALRHFDEGNCDTLKEILVTYNCCDDDYFNKKDWYDFVENPDILRVYANLGERVRQALLKTVQFCDAMRNAGIVGVL
TLDNQDLNGNWYDFGDFIQTTPGSGVPVVDSYYSLLMPILTLTRALTAESHVDTDLTKPYIKWDLLKYDFTEERLKLFDRYFKYWDQTYHPNCVNCLDDR
CILHCANFNVLFSTVFPPTSFGPLVRKIFVDGVPFVVSTGYHFRELGVVHNQDVNLHSSRLSFKELLVYAADPAMHAASGNLLLDKRTTCFSVAALTNNV
AFQTVKPGNFNKDFYDFAVSKGFFKEGSSVELKHFFFAQDGNAAISDYDYYRYNLPTMCDIRQLLFVVEVVDKYFDCYDGGCINANQVIVNNLDKSAGFP
FNKWGKARLYYDSMSYEDQDALFAYTKRNVIPTITQMNLKYAISAKNRARTVAGVSICSTMTNRQFHQKLLKSIAATRGATVVIGTSKFYGGWHNMLKTV
YSDVENPHLMGWDYPKCDRAMPNMLRIMASLVLARKHTTCCSLSHRFYRLANECAQVLSEMVMCGGSLYVKPGGTSSGDATTAYANSVFNICQAVTANVN
ALLSTDGNKIADKYVRNLQHRLYECLYRNRDVDTDFVNEFYAYLRKHFSMMILSDDAVVCFNSTYASQGLVASIKNFKSVLYYQNNVFMSEAKCWTETDL
TKGPHEFCSQHTMLVKQGDDYVYLPYPDPSRILGAGCFVDDIVKTDGTLMIERFVSLAIDAYPLTKHPNQEYADVFHLYLQYIRKLHDELTGHMLDMYSV
MLTNDNTSRYWEPEFYEAMYTPHTVLQ*
NGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLI
KNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPL
GAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQGP
LGSNRVCGVSAARLTPCGTGTSTDVVYRAFDIYNDKVAGFAKFLKTNCCRFQEKDEDDNLIDSYFVVKRHTFSNYQHEETIYNLLKDCPAVAKHDFFKFR
IDGDMVPHISRQRLTKYTMADLVYALRHFDEGNCDTLKEILVTYNCCDDDYFNKKDWYDFVENPDILRVYANLGERVRQALLKTVQFCDAMRNAGIVGVL
TLDNQDLNGNWYDFGDFIQTTPGSGVPVVDSYYSLLMPILTLTRALTAESHVDTDLTKPYIKWDLLKYDFTEERLKLFDRYFKYWDQTYHPNCVNCLDDR
CILHCANFNVLFSTVFPPTSFGPLVRKIFVDGVPFVVSTGYHFRELGVVHNQDVNLHSSRLSFKELLVYAADPAMHAASGNLLLDKRTTCFSVAALTNNV
AFQTVKPGNFNKDFYDFAVSKGFFKEGSSVELKHFFFAQDGNAAISDYDYYRYNLPTMCDIRQLLFVVEVVDKYFDCYDGGCINANQVIVNNLDKSAGFP
FNKWGKARLYYDSMSYEDQDALFAYTKRNVIPTITQMNLKYAISAKNRARTVAGVSICSTMTNRQFHQKLLKSIAATRGATVVIGTSKFYGGWHNMLKTV
YSDVENPHLMGWDYPKCDRAMPNMLRIMASLVLARKHTTCCSLSHRFYRLANECAQVLSEMVMCGGSLYVKPGGTSSGDATTAYANSVFNICQAVTANVN
ALLSTDGNKIADKYVRNLQHRLYECLYRNRDVDTDFVNEFYAYLRKHFSMMILSDDAVVCFNSTYASQGLVASIKNFKSVLYYQNNVFMSEAKCWTETDL
TKGPHEFCSQHTMLVKQGDDYVYLPYPDPSRILGAGCFVDDIVKTDGTLMIERFVSLAIDAYPLTKHPNQEYADVFHLYLQYIRKLHDELTGHMLDMYSV
MLTNDNTSRYWEPEFYEAMYTPHTVLQ*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Codon optimised for bacterial expression.
100042 R 1000 1001 1047 19007
Full Snapgene Map