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Plasmid
pGEX6P1 SARS-CoV-2 Nsp12 P919-Q924 Nsp13 6His
Parent Plasmid
pGEX6P1
Expressed
GST 3C SARS-CoV-2 Nsp12 P919-Q924 Nsp13 6His
DU Number
DU67899
Genbank
MN908947.3
Tag
GST
Species
SARS-CoV-2
Sequence of Insert
View Sequence
GGATCCCCGCATACCGTGCTGCAGGCGGTGGGCGCGTGCGTGCTGTGCAACAGCCAGACCAGCCTGCGCTGCGGCGCGTGCATTCGCCGCCCGTTTCTGT
GCTGCAAATGCTGCTATGATCATGTGATTAGCACCAGCCATAAACTGGTGCTGAGCGTGAACCCGTATGTGTGCAACGCGCCGGGCTGCGATGTGACCGA
TGTGACCCAGCTGTATCTGGGCGGCATGAGCTATTATTGCAAAAGCCATAAACCGCCGATTAGCTTTCCGCTGTGCGCGAACGGCCAGGTGTTTGGCCTG
TATAAAAACACCTGCGTGGGCAGCGATAACGTGACCGATTTTAACGCGATTGCGACCTGCGATTGGACCAACGCGGGCGATTATATTCTGGCGAACACCT
GCACCGAACGCCTGAAACTGTTTGCGGCGGAAACCCTGAAAGCGACCGAAGAAACCTTTAAACTGAGCTATGGCATTGCGACCGTGCGCGAAGTGCTGAG
CGATCGCGAACTGCATCTGAGCTGGGAAGTGGGCAAACCGCGCCCGCCGCTGAACCGCAACTATGTGTTTACCGGCTATCGCGTGACCAAAAACAGCAAA
GTGCAGATTGGCGAATATACCTTTGAAAAAGGCGATTATGGCGATGCGGTGGTGTATCGCGGCACCACCACCTATAAACTGAACGTGGGCGATTATTTTG
TGCTGACCAGCCATACCGTGATGCCGCTGAGCGCGCCGACCCTGGTGCCGCAGGAACATTATGTGCGCATTACCGGCCTGTATCCGACCCTGAACATTAG
CGATGAATTTAGCAGCAACGTGGCGAACTATCAGAAAGTGGGCATGCAGAAATATAGCACCCTGCAGGGCCCGCCGGGCACCGGCAAAAGCCATTTTGCG
ATTGGCCTGGCGCTGTATTATCCGAGCGCGCGCATTGTGTATACCGCGTGCAGCCATGCGGCGGTGGATGCGCTGTGCGAAAAAGCGCTGAAATATCTGC
CGATTGATAAATGCAGCCGCATTATTCCGGCGCGCGCGCGCGTGGAATGCTTTGATAAATTTAAAGTGAACAGCACCCTGGAACAGTATGTGTTTTGCAC
CGTGAACGCGCTGCCGGAAACCACCGCGGATATTGTGGTGTTTGATGAAATTAGCATGGCGACCAACTATGATCTGAGCGTGGTGAACGCGCGCCTGCGC
GCGAAACATTATGTGTATATTGGCGATCCGGCGCAGCTGCCGGCGCCGCGCACCCTGCTGACCAAAGGCACCCTGGAACCGGAATATTTTAACAGCGTGT
GCCGCCTGATGAAAACCATTGGCCCGGATATGTTTCTGGGCACCTGCCGCCGCTGCCCGGCGGAAATTGTGGATACCGTGAGCGCGCTGGTGTATGATAA
CAAACTGAAAGCGCATAAAGATAAAAGCGCGCAGTGCTTTAAAATGTTTTATAAAGGCGTGATTACCCATGATGTGAGCAGCGCGATTAACCGCCCGCAG
ATTGGCGTGGTGCGCGAATTTCTGACCCGCAACCCGGCGTGGCGCAAAGCGGTGTTTATTAGCCCGTATAACAGCCAGAACGCGGTGGCGAGCAAAATTC
TGGGCCTGCCGACCCAGACCGTGGATAGCAGCCAGGGCAGCGAATATGATTATGTGATTTTTACCCAGACCACCGAAACCGCGCATAGCTGCAACGTGAA
CCGCTTTAACGTGGCGATTACCCGCGCGAAAGTGGGCATTCTGTGCATTATGAGCGATCGCGATCTGTATGATAAACTGCAGTTTACCAGCCTGGAAATT
CCGCGCCGCAACGTGGCGACCCTGCAGGGACCCCATCATCATCATCATCATTGAGCGGCCGC
GCTGCAAATGCTGCTATGATCATGTGATTAGCACCAGCCATAAACTGGTGCTGAGCGTGAACCCGTATGTGTGCAACGCGCCGGGCTGCGATGTGACCGA
TGTGACCCAGCTGTATCTGGGCGGCATGAGCTATTATTGCAAAAGCCATAAACCGCCGATTAGCTTTCCGCTGTGCGCGAACGGCCAGGTGTTTGGCCTG
TATAAAAACACCTGCGTGGGCAGCGATAACGTGACCGATTTTAACGCGATTGCGACCTGCGATTGGACCAACGCGGGCGATTATATTCTGGCGAACACCT
GCACCGAACGCCTGAAACTGTTTGCGGCGGAAACCCTGAAAGCGACCGAAGAAACCTTTAAACTGAGCTATGGCATTGCGACCGTGCGCGAAGTGCTGAG
CGATCGCGAACTGCATCTGAGCTGGGAAGTGGGCAAACCGCGCCCGCCGCTGAACCGCAACTATGTGTTTACCGGCTATCGCGTGACCAAAAACAGCAAA
GTGCAGATTGGCGAATATACCTTTGAAAAAGGCGATTATGGCGATGCGGTGGTGTATCGCGGCACCACCACCTATAAACTGAACGTGGGCGATTATTTTG
TGCTGACCAGCCATACCGTGATGCCGCTGAGCGCGCCGACCCTGGTGCCGCAGGAACATTATGTGCGCATTACCGGCCTGTATCCGACCCTGAACATTAG
CGATGAATTTAGCAGCAACGTGGCGAACTATCAGAAAGTGGGCATGCAGAAATATAGCACCCTGCAGGGCCCGCCGGGCACCGGCAAAAGCCATTTTGCG
ATTGGCCTGGCGCTGTATTATCCGAGCGCGCGCATTGTGTATACCGCGTGCAGCCATGCGGCGGTGGATGCGCTGTGCGAAAAAGCGCTGAAATATCTGC
CGATTGATAAATGCAGCCGCATTATTCCGGCGCGCGCGCGCGTGGAATGCTTTGATAAATTTAAAGTGAACAGCACCCTGGAACAGTATGTGTTTTGCAC
CGTGAACGCGCTGCCGGAAACCACCGCGGATATTGTGGTGTTTGATGAAATTAGCATGGCGACCAACTATGATCTGAGCGTGGTGAACGCGCGCCTGCGC
GCGAAACATTATGTGTATATTGGCGATCCGGCGCAGCTGCCGGCGCCGCGCACCCTGCTGACCAAAGGCACCCTGGAACCGGAATATTTTAACAGCGTGT
GCCGCCTGATGAAAACCATTGGCCCGGATATGTTTCTGGGCACCTGCCGCCGCTGCCCGGCGGAAATTGTGGATACCGTGAGCGCGCTGGTGTATGATAA
CAAACTGAAAGCGCATAAAGATAAAAGCGCGCAGTGCTTTAAAATGTTTTATAAAGGCGTGATTACCCATGATGTGAGCAGCGCGATTAACCGCCCGCAG
ATTGGCGTGGTGCGCGAATTTCTGACCCGCAACCCGGCGTGGCGCAAAGCGGTGTTTATTAGCCCGTATAACAGCCAGAACGCGGTGGCGAGCAAAATTC
TGGGCCTGCCGACCCAGACCGTGGATAGCAGCCAGGGCAGCGAATATGATTATGTGATTTTTACCCAGACCACCGAAACCGCGCATAGCTGCAACGTGAA
CCGCTTTAACGTGGCGATTACCCGCGCGAAAGTGGGCATTCTGTGCATTATGAGCGATCGCGATCTGTATGATAAACTGCAGTTTACCAGCCTGGAAATT
CCGCGCCGCAACGTGGCGACCCTGCAGGGACCCCATCATCATCATCATCATTGAGCGGCCGC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPHTVLQAVGACVLCNSQTSLRCGACIRRPFLCCKCCYDHVISTSHKLVLSVNPYVCNAPGCDVTDVTQL
YLGGMSYYCKSHKPPISFPLCANGQVFGLYKNTCVGSDNVTDFNAIATCDWTNAGDYILANTCTERLKLFAAETLKATEETFKLSYGIATVREVLSDREL
HLSWEVGKPRPPLNRNYVFTGYRVTKNSKVQIGEYTFEKGDYGDAVVYRGTTTYKLNVGDYFVLTSHTVMPLSAPTLVPQEHYVRITGLYPTLNISDEFS
SNVANYQKVGMQKYSTLQGPPGTGKSHFAIGLALYYPSARIVYTACSHAAVDALCEKALKYLPIDKCSRIIPARARVECFDKFKVNSTLEQYVFCTVNAL
PETTADIVVFDEISMATNYDLSVVNARLRAKHYVYIGDPAQLPAPRTLLTKGTLEPEYFNSVCRLMKTIGPDMFLGTCRRCPAEIVDTVSALVYDNKLKA
HKDKSAQCFKMFYKGVITHDVSSAINRPQIGVVREFLTRNPAWRKAVFISPYNSQNAVASKILGLPTQTVDSSQGSEYDYVIFTQTTETAHSCNVNRFNV
AITRAKVGILCIMSDRDLYDKLQFTSLEIPRRNVATLQGPHHHHHH*
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPHTVLQAVGACVLCNSQTSLRCGACIRRPFLCCKCCYDHVISTSHKLVLSVNPYVCNAPGCDVTDVTQL
YLGGMSYYCKSHKPPISFPLCANGQVFGLYKNTCVGSDNVTDFNAIATCDWTNAGDYILANTCTERLKLFAAETLKATEETFKLSYGIATVREVLSDREL
HLSWEVGKPRPPLNRNYVFTGYRVTKNSKVQIGEYTFEKGDYGDAVVYRGTTTYKLNVGDYFVLTSHTVMPLSAPTLVPQEHYVRITGLYPTLNISDEFS
SNVANYQKVGMQKYSTLQGPPGTGKSHFAIGLALYYPSARIVYTACSHAAVDALCEKALKYLPIDKCSRIIPARARVECFDKFKVNSTLEQYVFCTVNAL
PETTADIVVFDEISMATNYDLSVVNARLRAKHYVYIGDPAQLPAPRTLLTKGTLEPEYFNSVCRLMKTIGPDMFLGTCRRCPAEIVDTVSALVYDNKLKA
HKDKSAQCFKMFYKGVITHDVSSAINRPQIGVVREFLTRNPAWRKAVFISPYNSQNAVASKILGLPTQTVDSSQGSEYDYVIFTQTTETAHSCNVNRFNV
AITRAKVGILCIMSDRDLYDKLQFTSLEIPRRNVATLQGPHHHHHH*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Codon optimised for bacterial expression.
100042 R 1000 1001 1047 19007
Full Snapgene Map