SARS-CoV-2 Nsp3

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Plasmid
pTYB2 Nsp3 A1-N1329 Linker D1584-G1945 Intein CBD
Parent Plasmid
pTYB2
Expressed
Nsp3 A1-N1329 Linker D1584-G1945 Intein CBD
DU Number
DU70078
Genbank
MN908947.3
Species
SARS-CoV-2
Sequence of Insert
CATATGGCCCCTACCAAGGTGACCTTCGGAGACGACACCGTGATCGAGGTGCAGGGTTACAAGAGCGTGAACATCACCTTCGAGCTGGACGAACGCATCGACAAGGTGCTGAACGAAAAGTGCTCCGCTTACACCGTGGAGCTGGGCACCGAGGTGAACGAATTCGCCTGCGTGGTGGCCGACGCTGTGATCAAGACCCTGCAGCCAGTGTCCGAACTGCTGACCCCTCTGGGTATCGACCTGGACGAGTGGTCTATGGCTACCTACTACCTGTTCGACGAGAGCGGCGAATTCAAGCTGGCCTCTCACATGTACTGCAGCTTCTACCCTCCCGACGAAGACGAGGAAGAGGGTGACTGCGAAGAGGAAGAGTTCGAGCCTTCCACTCAGTACGAGTACGGCACCGAAGACGACTACCAGGGCAAGCCTCTGGAATTCGGAGCCACCTCTGCCGCTCTGCAGCCTGAAGAGGAACAGGAGGAAGACTGGCTGGACGACGACTCCCAGCAGACCGTGGGTCAGCAGGACGGCTCTGAAGACAACCAGACCACCACCATCCAGACCATCGTGGAGGTGCAGCCTCAGCTGGAGATGGAACTGACCCCAGTGGTGCAGACCATCGAGGTGAACAGCTTCTCCGGCTACCTGAAGCTGACCGACAACGTGTACATCAAGAACGCTGACATCGTGGAGGAAGCCAAGAAGGTGAAACCAACCGTGGTGGTGAACGCCGCTAACGTGTACCTGAAGCACGGCGGAGGTGTGGCTGGAGCTCTGAACAAGGCTACCAACAACGCCATGCAGGTGGAATCCGACGACTACATCGCTACCAACGGTCCTCTGAAGGTGGGCGGATCCTGCGTGCTGTCTGGCCACAACCTGGCCAAGCACTGCCTGCACGTGGTGGGACCAAACGTGAACAAGGGAGAGGACATCCAGCTGCTGAAGAGCGCCTACGAAAACTTCAACCAGCACGAGGTGCTGCTGGCTCCACTGCTGTCTGCCGGAATCTTCGGTGCTGATCCCATCCACAGCCTGCGCGTGTGCGTGGACACCGTGAGGACCAACGTGTACCTGGCCGTGTTCGACAAGAACCTGTACGACAAGCTGGTGAGCTCCTTCCTGGAGATGAAGTCCGAAAAGCAGGTGGAGCAGAAGATCGCCGAGATCCCCAAGGAGGAAGTGAAGCCTTTCATCACCGAATCTAAGCCCAGCGTGGAGCAGAGAAAGCAGGACGACAAGAAGATCAAGGCCTGCGTGGAGGAAGTGACCACCACCCTGGAGGAAACCAAGTTCCTGACCGAGAACCTGCTGCTGTACATCGACATCAACGGCAACCTGCACCCTGACTCCGCTACCCTGGTGTCTGACATCGACATCACCTTCCTGAAGAAGGACGCCCCCTACATCGTGGGCGACGTGGTGCAGGAAGGAGTGCTGACCGCTGTGGTCATCCCTACCAAGAAGGCCGGTGGCACCACCGAGATGCTGGCCAAGGCTCTGCGTAAGGTGCCAACCGACAACTACATCACCACCTACCCCGGTCAGGGCCTGAACGGTTACACCGTGGAGGAAGCTAAGACCGTGCTGAAGAAGTGCAAGTCTGCCTTCTACATCCTGCCCTCCATCATCTCTAACGAGAAGCAGGAAATCCTGGGCACCGTGAGCTGGAACCTGCGCGAAATGCTGGCCCACGCTGAGGAAACCAGGAAGCTGATGCCCGTGTGCGTGGAGACCAAGGCCATCGTGAGCACCATCCAGAGAAAGTACAAGGGCATCAAGATCCAGGAGGGTGTGGTGGACTACGGCGCTCGTTTCTACTTCTACACCTCCAAGACCACCGTGGCCTCTCTGATCAACACCCTGAACGACCTGAACGAGACCCTGGTGACCATGCCACTGGGATACGTGACCCACGGTCTGAACCTGGAGGAAGCCGCTAGATACATGCGTTCCCTGAAGGTGCCTGCTACCGTGAGCGTGAGCAGCCCTGACGCTGTGACCGCCTACAACGGATACCTGACCTCCTCTAGCAAGACCCCTGAGGAACACTTCATCGAAACCATCAGCCTGGCCGGTTCCTACAAGGACTGGTCTTACAGCGGACAGTCCACCCAGCTGGGCATCGAGTTCCTGAAGCGCGGAGACAAGAGCGTGTACTACACCTCCAACCCAACCACCTTCCACCTGGACGGTGAAGTGATCACCTTCGACAACCTGAAGACCCTGCTGTCTCTGCGCGAGGTGAGGACCATCAAGGTGTTCACCACCGTGGACAACATCAACCTGCACACCCAGGTGGTGGACATGAGCATGACCTACGGACAGCAGTTCGGTCCTACCTACCTGGACGGAGCTGACGTGACCAAGATCAAGCCACACAACTCCCACGAGGGCAAGACCTTCTACGTGCTGCCTAACGATGACACCCTGAGAGTGGAGGCTTTCGAATACTACCACACCACCGACCCATCCTTCCTGGGCAGGTACATGTCTGCCCTGAACCACACCAAGAAGTGGAAGTACCCCCAGGTGAACGGACTGACCAGCATCAAGTGGGCCGACAACAACTGCTACCTGGCCACCGCTCTGCTGACCCTGCAGCAGATCGAACTGAAGTTCAACCCACCCGCTCTGCAGGACGCTTACTACAGAGCCCGTGCTGGCGAGGCCGCTAACTTCTGCGCTCTGATCCTGGCCTACTGCAACAAGACCGTGGGCGAACTGGGAGACGTGAGAGAGACCATGAGCTACCTGTTCCAGCACGCCAACCTGGACTCCTGCAAGCGTGTGCTGAACGTGGTGTGCAAGACCTGCGGCCAGCAGCAGACCACCCTGAAGGGAGTGGAAGCTGTGATGTACATGGGTACCCTGTCCTACGAGCAGTTCAAGAAGGGCGTGCAGATCCCTTGCACCTGCGGAAAGCAGGCCACCAAGTACCTGGTGCAGCAGGAAAGCCCATTCGTGATGATGTCCGCTCCTCCAGCCCAGTACGAACTGAAGCACGGCACCTTCACCTGCGCTTCCGAGTACACCGGCAACTACCAGTGCGGACACTACAAGCACATCACCTCTAAGGAGACCCTGTACTGCATCGACGGAGCCCTGCTGACCAAGTCCTCTGAATACAAGGGTCCCATCACCGACGTGTTCTACAAGGAGAACAGCTACACCACCACCATCAAGCCCGTGACCTACAAGCTGGACGGCGTGGTGTGCACCGAAATCGACCCTAAGCTGGACAACTACTACAAGAAGGACAACTCTTACTTCACCGAGCAGCCTATCGACCTGGTGCCAAACCAGCCCTACCCTAACGCCAGCTTCGACAACTTCAAGTTCGTGTGCGACAACATCAAGTTCGCTGACGACCTGAACCAGCTGACCGGATACAAGAAGCCTGCCTCTAGAGAGCTGAAGGTGACCTTCTTCCCAGACCTGAACGGCGACGTGGTGGCTATCGACTACAAGCACTACACCCCAAGCTTCAAGAAGGGAGCCAAGCTGCTGCACAAGCCCATCGTGTGGCACGTGAACAACGCTACCAACAAGGCCACCTACAAGCCTAACACCTGGTGCATCCGTTGCCTGTGGTCTACCAAGCCAGTGGAAACCTCCAACTCTTTCGACGTGCTGAAGAGCGAGGACGCTCAGGGCATGGACAACCTGGCCTGCGAAGACCTGAAGCCCGTGAGCGAGGAAGTGGTGGAGAACCCAACCATCCAGAAGGACGTGCTGGAATGCAACGTGAAGACCACCGAGGTGGTGGGAGACATCATCCTGAAGCCAGCTAACAACAGCCTGAAGATCACCGAGGAAGTGGGTCACACCGACCTGATGGCCGCTTACGTGGACAACAGCTCCCTGACCATCAAGAAGCCCAACGAGCTGAGCAGGGTGCTGGGTCTGAAGACCCTGGCTACTCACGGTCTGGCTGCTGTGAACTCTGTGCCATGGGACACCATCGCTAACTACGCCAAGCCTTTCCTGAACggtagtGGAGGgTCAggaTCAGGAggtTCCagtGGTgggtcaggaGACGGTTGCAACTCCTCTACCTGCATGATGTGCTACAAGCGCAACAGGGCTACCAGGGTGGAGTGCACCACCATCGTGAACGGCGTGCGCAGGTCCTTCTACGTGTACGCCAACGGCGGCAAGGGATTCTGCAAGCTGCACAACTGGAACTGCGTGAACTGCGACACCTTCTGCGCTGGCTCCACCTTCATCTCTGACGAGGTGGCCAGAGACCTGAGCCTGCAGTTCAAGCGTCCAATCAACCCCACCGACCAGAGCTCCTACATCGTGGACTCTGTGACCGTGAAGAACGGTAGCATCCACCTGTACTTCGACAAGGCTGGCCAGAAGACCTACGAGCGCCACTCTCTGAGCCACTTCGTGAACCTGGACAACCTGAGGGCCAACAACACCAAGGGCAGCCTGCCTATCAACGTGATCGTGTTCGACGGAAAGTCCAAGTGCGAGGAATCTAGCGCCAAGTCCGCCAGCGTGTACTACAGCCAGCTGATGTGCCAGCCAATCCTGCTGCTGGACCAGGCTCTGGTGTCTGACGTGGGAGACAGCGCCGAAGTGGCCGTGAAGATGTTCGACGCCTACGTGAACACCTTCTCCTCTACCTTCAACGTGCCTATGGAGAAGCTGAAGACCCTGGTGGCTACTGCTGAGGCTGAACTGGCTAAGAACGTGTCCCTGGACAACGTGCTGAGCACCTTCATCTCCGCCGCTAGGCAGGGTTTCGTGGACTCTGACGTGGAAACCAAAGACGTGGTGGAGTGCCTGAAGCTGTCTCACCAGAGCGACATCGAAGTGACCGGCGACTCCTGCAACAACTACATGCTGACCTACAACAAGGTGGAGAACATGACCCCAAGAGACCTGGGAGCTTGCATCGACTGCTCCGCCCGTCACATCAACGCTCAGGTGGCCAAGTCTCACAACATCGCCCTGATCTGGAACGTGAAGGACTTCATGAGCCTGTCCGAGCAGCTGCGCAAGCAGATCAGGAGCGCCGCTAAGAAGAACAACCTGCCCTTCAAGCTGACCTGCGCTACCACCAGACAGGTGGTGAACGTGGTGACCACCAAGATCGCCCTGAAGGGCGGACTCGAG
Amino Acid Sequence
MAPTKVTFGDDTVIEVQGYKSVNITFELDERIDKVLNEKCSAYTVELGTEVNEFACVVADAVIKTLQPVSELLTPLGIDLDEWSMATYYLFDESGEFKLA
SHMYCSFYPPDEDEEEGDCEEEEFEPSTQYEYGTEDDYQGKPLEFGATSAALQPEEEQEEDWLDDDSQQTVGQQDGSEDNQTTTIQTIVEVQPQLEMELT
PVVQTIEVNSFSGYLKLTDNVYIKNADIVEEAKKVKPTVVVNAANVYLKHGGGVAGALNKATNNAMQVESDDYIATNGPLKVGGSCVLSGHNLAKHCLHV
VGPNVNKGEDIQLLKSAYENFNQHEVLLAPLLSAGIFGADPIHSLRVCVDTVRTNVYLAVFDKNLYDKLVSSFLEMKSEKQVEQKIAEIPKEEVKPFITE
SKPSVEQRKQDDKKIKACVEEVTTTLEETKFLTENLLLYIDINGNLHPDSATLVSDIDITFLKKDAPYIVGDVVQEGVLTAVVIPTKKAGGTTEMLAKAL
RKVPTDNYITTYPGQGLNGYTVEEAKTVLKKCKSAFYILPSIISNEKQEILGTVSWNLREMLAHAEETRKLMPVCVETKAIVSTIQRKYKGIKIQEGVVD
YGARFYFYTSKTTVASLINTLNDLNETLVTMPLGYVTHGLNLEEAARYMRSLKVPATVSVSSPDAVTAYNGYLTSSSKTPEEHFIETISLAGSYKDWSYS
GQSTQLGIEFLKRGDKSVYYTSNPTTFHLDGEVITFDNLKTLLSLREVRTIKVFTTVDNINLHTQVVDMSMTYGQQFGPTYLDGADVTKIKPHNSHEGKT
FYVLPNDDTLRVEAFEYYHTTDPSFLGRYMSALNHTKKWKYPQVNGLTSIKWADNNCYLATALLTLQQIELKFNPPALQDAYYRARAGEAANFCALILAY
CNKTVGELGDVRETMSYLFQHANLDSCKRVLNVVCKTCGQQQTTLKGVEAVMYMGTLSYEQFKKGVQIPCTCGKQATKYLVQQESPFVMMSAPPAQYELK
HGTFTCASEYTGNYQCGHYKHITSKETLYCIDGALLTKSSEYKGPITDVFYKENSYTTTIKPVTYKLDGVVCTEIDPKLDNYYKKDNSYFTEQPIDLVPN
QPYPNASFDNFKFVCDNIKFADDLNQLTGYKKPASRELKVTFFPDLNGDVVAIDYKHYTPSFKKGAKLLHKPIVWHVNNATNKATYKPNTWCIRCLWSTK
PVETSNSFDVLKSEDAQGMDNLACEDLKPVSEEVVENPTIQKDVLECNVKTTEVVGDIILKPANNSLKITEEVGHTDLMAAYVDNSSLTIKKPNELSRVL
GLKTLATHGLAAVNSVPWDTIANYAKPFLNGSGGSGSGGSSGGSGDGCNSSTCMMCYKRNRATRVECTTIVNGVRRSFYVYANGGKGFCKLHNWNCVNCD
TFCAGSTFISDEVARDLSLQFKRPINPTDQSSYIVDSVTVKNGSIHLYFDKAGQKTYERHSLSHFVNLDNLRANNTKGSLPINVIVFDGKSKCEESSAKS
ASVYYSQLMCQPILLLDQALVSDVGDSAEVAVKMFDAYVNTFSSTFNVPMEKLKTLVATAEAELAKNVSLDNVLSTFISAARQGFVDSDVETKDVVECLK
LSHQSDIEVTGDSCNNYMLTYNKVENMTPRDLGACIDCSARHINAQVAKSHNIALIWNVKDFMSLSEQLRKQIRSAAKKNNLPFKLTCATTRQVVNVVTT
KIALKGGLEPGCFAKGTNVLMADGSIECIENIEVGNKVMGKDGRPREVIKLPRGRETMYSVVQKSQHRAHKSDSSREVPELLKFTCNATHELVVRTPRSV
RRLSRTIKGVEYFEVITFEMGQKKAPDGRIVELVKEVSKSYPISEGPERANELVESYRKASNKAYFEWTIEARDLSLLGSHVRKATYQTYAPILYENDHF
FDYMQKSKFHLTIEGPKVLAYLLGLWIGDGLSDRATFSVDSRDTSLMERVTEYAEKLNLCAEYKDRKEPQVAKTVNLYSKVVRGNGIRNNLNTENPLWDA
IVGLGFLKDGVKNIPSFLSTDNIGTRETFLAGLIDSDGYVTDEHGIKATIKTIHTSVRDGLVSLARSLGLVVSVNAEPAKVDMNVTKHKISYAIYMSGGD
VLLNVLSKCAGSKKFRPAPAAAFARECRGFYFELQELKEDDYYGITLSDDSDHQFLLGSQVVVHACGGLTGLNSGLTTNPGVSAWQVNTAYTAGQLVTYN
GKTYKCLQPHTSLAGWEPSNVPALWQLQ*
Vector Type
Bacterial
Antibiotic
Amp
100042 R 1000 1001 1047 19007